Extraction and characterization of steviol glycosides from Stevia rebaudiana bertoni leaves
نویسندگان
چکیده
Steviol glycosides are highly sweet diterpene glycosides found in the Paragyan shrub, Stevia rebaudiana Bertoni. Steviol glycosides, mainly stevioside and rebaudioside, principal components of Stevia rebaudiana leaf, has become well known for their sweetness and are used as non-caloric sweeteners in several countries due to their nutritional and pharmacological benefits. Hence, craving for sweetness led man to discover several forms of alternative sweeteners. In this study, different polar and non-polar solvents were used for extracting glycosides from stevia leaves and the components isolated through column chromatography were characterized as steviolmonoside, stevioside and rebaudioside B on the basis of spectral (IR and 1H-NMR data), physical and chemical characteristics.
منابع مشابه
Optimization of ultrasound assisted extraction of functional ingredients from Stevia rebaudiana Bertoni leaves
*Corresponding author e-mail: [email protected] A b s t r a c t. The aim of the present study was to reveal an effective extraction procedure for maximization of the yield of steviol glycosides and total phenolic compounds as well as antioxidant activity in stevia extracts. Ultrasound assisted extraction was compared with conventional solvent extraction. The examined solvents were water (100oC/24 ...
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The aim of this study was to learn the interactions among the endophytic bacteria, the plant growth, the foliar spray of fulvic acid, and the accumulation of steviol glycosides in the leaves of Stevia rebaudiana. Metagenomic DNA was extracted from the Stevia leaves at different growth stages with or without the fulvic acid treatment; and the diversity of endophytic bacteria in Stevia leaves was...
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The complete proton and carbon NMR spectral assignments of a diterpene glycoside isolated from the commercial extract of the leaves of Stevia rebaudiana Bertoni, 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2-O-α-L-rhamnopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester] (1); also known as rebaudioside N, was achieved by the ex...
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The most important characteristic of stevia is its high sweetness with zero calories which is due to the presence of Steviol glycosides (SVglys). This research aims to address the effect of salicylic acid (SA) and microelements viz. iron (Fe) and zinc (Zn) under different soil water potentials (-0.5, -3.5, -6.5 and -10 atm) on the production of SVglys and total sugar content in the leaves o...
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